Reprogramming of somatic cells and nuclei by Xenopus oocyte and egg extracts
نویسندگان
چکیده
منابع مشابه
Patterns of DNA replication in Drosophila polytene nuclei replicating in Xenopus egg and oocyte extracts.
We have used Xenopus laevis cell-free extracts to study patterns of DNA replication in polytene nuclei isolated from salivary glands of Drosophila melanogaster 3rd instar larvae. Replication was visualized by supplementation with biotin-dUTP so that nascent DNA became labelled, thus allowing detection with fluorescein or Texas-Red-conjugated streptavidin. Biotin incorporation was dependent on i...
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Transfer of somatic cell nuclei to enucleated eggs and ectopic expression of specific transcription factors are two different reprogramming strategies used to generate pluripotent cells from differentiated cells. However, these methods are poorly efficient, and other unknown factors might be required to increase their success rate. Here we show that Xenopus egg extracts at the metaphase stage (...
متن کاملEfficient reactivation of Xenopus erythrocyte nuclei in Xenopus egg extracts.
Rapid genome replication is one of the hallmarks of the frog embryonic cell cycle. We report here that complete reactivation of quiescent somatic cell nuclei in Xenopus egg extracts depends on prior restructuring of the nuclear substrate and prior preparation of cytoplasmic extract with the highest capacity to initiate and sustain DNA synthesis. Nuclei from mature erythrocytes swell, replicate ...
متن کاملNuclear Reprogramming of Human Somatic Cells by Xenopus Egg Extract Requires BRG1
Animal cloning by nuclear transplantation in amphibia was demonstrated almost half a century ago and raised the question of the mechanisms and genes involved in nuclear reprogramming. Here, we demonstrate nuclear reprogramming of permeabilized human cells using extracts from Xenopus laevis eggs and early embryos. We show upregulation of pluripotency markers Oct-4 and germ cell alkaline phosphat...
متن کاملMovement of nuclei along microtubules in Xenopus egg extracts
Microtubules are implicated in the movement and positioning of nuclei in many cell types. Nuclei can be moved by forces acting on microtubules nucleated at the spindle pole body, as in fungi [1], or microtubules nucleated at the centrosome, as during migration of the male (sperm) pronucleus towards the centre of the zygote after fertilization [2] [3] [4]. The dramatic movements of the female pr...
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ژورنال
عنوان ژورنال: The International Journal of Developmental Biology
سال: 2016
ISSN: 0214-6282
DOI: 10.1387/ijdb.160163at